Screening and Purification of a Chymotrypsin Inhibitor from Entrolobium Saman Seeds
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چکیده
منابع مشابه
Genomic and cDNA cloning, expression, purification, and characterization of chymotrypsin-trypsin inhibitor from winged bean seeds.
A 516-bp winged bean chymotrypsin-trypsin inhibitor (WbCTI) gene was amplified from genomic DNA and cDNA isolated from winged bean using a pair of degenerate primers designed on the basis of the amino acid sequences of WbCTI. The amplified PCR products were cloned and sequenced to confirm their authenticity. DNA sequence analysis of the genomic and cDNA clones of WbCTI revealed the same nucleot...
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Protease inhibitors were extracted from the seeds of Lavatera cashmeriana Camb by ammonium sulphate precipitation and purified by chromatography on DEAE-cellulose and Sephadex G-100 column. The bound protein eluted into four major peaks which we named as LC-pi I, II, III and IV, all showing strong anti protease activity against trypsin, chymotrypsin and elastase. LC-pi I was screened for antiba...
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The serine proteinase inhibitor from barley seeds, chymotrypsin inhibitor 2(CI-2), has been crystallized in a molecular complex with subtilisin Novo (EC 3.4.21.14). The crystal structure of this complex has been determined at 2.1-A resolution by the molecular replacement method and partially refined by restrained-parameter least-squares methods. The present crystallographic R factor (SigmaFo[un...
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inhibiting subtilisin BPN'. This paper describes purification and characterization of a subtilisin inhibitor (FMSI) from foxtail millet grain. Protein was measured by the method of Lowry et al.2) using bovine serum albumin as a standard. Protein concentrations of enzyme solutions were measured spectrophotometrically with extinction coefficients as described previously.3) The concentration of St...
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A protein proteinase inhibitor was purified from a seed extract of amaranth (Amaranthus hypochondriacus) by precipitation with (NH4)2SO4, gel-filtration chromatography, ion-exchange chromatography, and reverse-phase high-performance liquid chromatography. It is a 69-amino acid protein with a high content of valine, arginine, and glutamic acid, but lacking in methionine. The inhibitor has a rela...
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ژورنال
عنوان ژورنال: Sultan Qaboos University Journal for Science [SQUJS]
سال: 2010
ISSN: 2414-536X,1027-524X
DOI: 10.24200/squjs.vol15iss0pp19-29